soluble rage (srage) and hmgb1  (MyBiosource Biotechnology)

Journal: Scientific Reports

Article Title: The trajectory of osteoblast progenitor cells in patients with type 2 diabetes and the predictive model for their osteogenic differentiation ability

doi: 10.1038/s41598-023-29677-8

Figure Lengend Snippet: Clinical characteristics of the study participants.

Article Snippet: Venous blood (35–40 mL) was collected from all enrolled participants to isolate the PBMC and determine serum levels of interleukin 1-β (IL1-β) (R&D, Minneapolis, MN, USA), pentosidine (Elabscience Biotechnology, WuHan, Hubei, China), soluble RAGE (sRAGE) (R&D, Minneapolis, MN, USA), and tumor necrosis factor-α (TNF-α) (R&D, Minneapolis, MN, USA) by ELISA.

Techniques:

Journal: Scientific Reports

Article Title: The trajectory of osteoblast progenitor cells in patients with type 2 diabetes and the predictive model for their osteogenic differentiation ability

doi: 10.1038/s41598-023-29677-8

Figure Lengend Snippet: Clinical characteristics of the study participants with type 2 diabetes.

Article Snippet: Venous blood (35–40 mL) was collected from all enrolled participants to isolate the PBMC and determine serum levels of interleukin 1-β (IL1-β) (R&D, Minneapolis, MN, USA), pentosidine (Elabscience Biotechnology, WuHan, Hubei, China), soluble RAGE (sRAGE) (R&D, Minneapolis, MN, USA), and tumor necrosis factor-α (TNF-α) (R&D, Minneapolis, MN, USA) by ELISA.

Techniques:

Journal: Cells

Article Title: Replacement of Lost Substance P Reduces Fibrosis in the Diabetic Heart by Preventing Adverse Fibroblast and Macrophage Phenotype Changes

doi: 10.3390/cells10102659

Figure Lengend Snippet: SP reduces RAGE and oxidative stress while increasing nitric oxide in isolated cardiac fibroblasts in response to high glucose. ( A ) Receptor for advanced glycation end product (RAGE) in cardiac fibroblast lysates; ( B ) soluble RAGE (sRAGE); and ( C ) hydrogen peroxide (H 2 O 2 ) in the culture media of isolated cardiac fibroblasts in response to normal glucose (control, 5 mM), high glucose (HG, 25 mM), and HG with increasing concentrations of SP (10 to 1000 nM); ( D ) superoxide dismutase levels in cardiac fibroblast lysates in response to control, HG, and HG with increasing concentrations of SP; ( E ) total nitrate/nitrite as a marker of nitric oxide (NO) production in the culture media of isolated cardiac fibroblasts in response to control, HG, and HG with increasing concentrations of SP. SP alters cytokine production by isolated cardiac fibroblasts in response to high glucose. ( F ) TNF-α, ( G ) CCL2, ( H ) IL-4, ( I ) IL-6, and ( J ) IL-10 levels in isolated cardiac fibroblast cell culture media in response to normal glucose (control, 5 mM), high glucose (HG, 25 mM), and HG with increasing concentrations of SP (10 to 1000 nM). Data are expressed as mean ± SEM and were analyzed by one-way ANOVA with Tukey post hoc test; * p < 0.05 vs. control, ** p < 0.01 vs. control, **** p < 0.0001 vs. control, † p < 0.05 vs. HG, †† p < 0.01 vs. HG, ††† p < 0.001 vs. HG. n = 4–6 for control and n = 5–6 for all other groups.

Article Snippet: Lysyl oxidase (LOX, MyBioSource, San Diego, CA, USA), bone morphogenic protein 1 (BMP-1, Novus Biologicals, Littleton, CO, USA), soluble RAGE (sRAGE, MyBioSource, San Diego, CA, USA), hydrogen peroxide (H 2 O 2, Cell BioLabs Inc, San Diego, CA, USA), superoxide dismutase (Cell BioLabs Inc, San Diego, CA, USA), and nitrate/nitrite (nitric oxide, NO, Cell BioLabs Inc, San Diego, CA, USA) were measured in fibroblast media.

Techniques: Isolation, Marker, Cell Culture